Percorrer por autor "Datta, Abhishek"
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- Electrical Stimulation Optimization in Bioreactors for Tissue Engineering ApplicationsPublication . Pascoal-Faria, Paula; Ferreira, Pedro Castelo; Datta, Abhishek; Amado, Sandra; Moura, Carla; Alves, NunoWe review here the current research status on bioreactors for tissue engineering with cell electrical stimulation. Depending on the cell types, electrical stimulation has distinct objectives: 1) being employed both to mimic and enhance endogenous electricity measured in the natural regeneration of living organisms and 2) to mimic strain working conditions for contractible tissues (for instance muscle and cardiac tissues). Understanding the distinct parameters involved in electrical stimulation is crucial to optimize its application. The results presented in the literature and reviewed here reveal that the application of electrical stimulation can be essential for tissue engineering applications.
- High-resolution head model of transcranial direct current stimulation: A labeling analysisPublication . Thomas, Chris; Huang, Yu; Faria, Paula Cristina; Datta, AbhishekThe ability of transcranial direct current stimulation (tDCS) to produce lasting polarity-specific modulatory effects continues to drive use both in research and clinical domains. Computational models of tDCS over the years have provided valuable insight on the current flow pattern and magnitude of electric field induced in the cortex. However, induced cortical values are usually not systematically quantified for different brain subcomponents that allow further investigation into the relevant contribution of these distinct regions. This information is of significant interest given different subcomponents of the brain contribute to different functions that ultimately underlie net outcomes. Thus given a particular stimulation response and the current flow pattern, one can potentially infer results in relation to current flow in different compartments (regions affected/spared, magnitude, etc.) The aim of this study is to determine tDCS induced electric field/current density using a high resolution head model incorporating a brain parcellated into 17 notable gyri and 10 sub-cortical regions. We consider both conventional tDCS and High Definition (HD)-tDCS electrode montages. The induced electrical field in each parcellated brain region is computed and compared across the two montages.Findings indicate that maximum electrical field is induced in the precentral gyrus for both the montages considered. As expected, the current flow pattern using the HD-tDCS montage considered is more restricted- both spatially and depth-wise. The conventional tDCS montage results in deeper current flow with sub-cortical structures subject to as much as 47-95% the current flow in the upper cortical regions. For the HD montage, electric field in the subcortical structures drop to 12-32% of the values induced in the upper regions. Incorporation of labeled human head models may guide rational electrode design and optimization of tDCS by providing more detailed and systematic information.
- A multimodal stimulation cell culture bioreactor for tissue engineering: A numerical modelling approachPublication . Meneses, João; Silva, João C.; Fernandes, Sofia R.; Datta, Abhishek; Ferreira, Frederico Castelo; Moura, Carla; Amado, Sandra; Alves, Nuno; Pascoal-Faria, PaulaThe use of digital twins in tissue engineering (TE) applications is of paramount importance to reduce the number of in vitro and in vivo tests. To pursue this aim, a novel multimodal bioreactor is developed, combining 3D design with numerical stimulation. This approach will facilitate the reproducibility between studies and the platforms optimisation (physical and digital) to enhance TE. The new bioreactor was specifically designed to be additive manufactured, which could not be reproduced with conventional techniques. Specifically, the design suggested allows the application of dual stimulation (electrical and mechanical) of a scaffold cell culture. For the selection of the most appropriate material for bioreactor manufacturing several materials were assessed for their cytotoxicity. Numerical modelling methods were then applied to the new bioreactor using one of the most appropriate material (Polyethylene Terephthalate Glycol-modified (PETG)) to find the optimal stimulation input parameters for bone TE based on two reported in vitro studies.