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- 3D Photo-Fabrication for Tissue Engineering and Drug DeliveryPublication . Brás Pereira, Rúben Filipe; Bartolo, PauloThe most promising strategies in tissue engineering involve the integration of a triad of biomaterials, living cells, and biologically active molecules to engineer synthetic environments that closely mimic the healing milieu present in human tissues, and that stimulate tissue repair and regeneration. To be clinically effective, these environments must replicate, as closely as possible, the main characteristics of the native extracellular matrix (ECM) on a cellular and subcellular scale. Photo-fabrication techniques have already been used to generate 3D environments with precise architectures and heterogeneous composition, through a multi-layer procedure involving the selective photocrosslinking reaction of a light-sensitive prepolymer. Cells and therapeutic molecules can be included in the initial hydrogel precursor solution, and processed into 3D constructs. Recently, photo-fabrication has also been explored to dynamically modulate hydrogel features in real time, providing enhanced control of cell fate and delivery of bioactive compounds. This paper focuses on the use of 3D photo-fabrication techniques to produce advanced constructs for tissue regeneration and drug delivery applications. State-of-the-art photo-fabrication techniques are described, with emphasis on the operating principles and biofabrication strategies to create spatially controlled patterns of cells and bioactive factors. Considering its fast processing, spatiotemporal control, high resolution, and accuracy, photo-fabrication is assuming a critical role in the design of sophisticated 3D constructs. This technology is capable of providing appropriate environments for tissue regeneration, and regulating the spatiotemporal delivery of therapeutics.
- Current Strategies to Produce 3D Electrospun-Based Wound Dressings for Skin RegenerationPublication . Ferreira, Carolina A. M.; Lemos, Marco F. L.; Maurício, Ana Colette; Dias, Juliana R.Tissue engineering-based wound dressings are a promising treatment approach that mimics the native skin microenvironment to promote effective healing and tissue regeneration. In fact, those advanced wound dressings can be made by electrospinning which has revolutionized the field of wound healing by developing biostructures that closely mimic the skin’s extracellular matrix (ECM). To date, most electrospinning research has focused on composition and materials rather than exploring advanced deposition strategies. Conventional electrospinning using random, aligned fibers, co-electrospinning, core-shell approaches, or redesigned collectors has achieved significant results in wound healing, which are discussed in this article. However, these approaches lack the development of realistic 3D structures. To achieve that, advancing approaches through the combination of electrospinning with different techniques such as gas foaming, short nanofiber assembly, or 3D printing significantly enhance skin regeneration of deep wounds (full-thickness and chronic wounds) compared to 2D structures. This review highlights the latest developments, design principles, and recent breakthroughs in electrospinning structures for skin regeneration and provides a fresh perspective for upcoming research in the field of 3D electrospun-based structures.
- Design of a Video Otoscope Prototype with an Integrated Scanner for Hearing Aid Direct Digital Manufacturing: A Preliminary StudyPublication . Ganhão, Francisco; Santos, António Carvalho; Silva, Carla; Monteiro de Moura, Carla SofiaIn the current landscape of hearing rehabilitation, ear mold manufacturing typically involves the injection of silicone into the external ear canal (EEC) of each patient. This invasive procedure poses several risks, including the potential for silicone residue retention and tympanic membrane perforation, which may necessitate surgical intervention. To mitigate these risks, we present the design of a video otoscope that integrates a scanner capable of capturing high-precision, real-time images of the EEC’s geometry. The developed device allows (i) the generation of a 3D CAD model leading to the direct, quick, and low-cost production of customized hearing aids using 3D printing and (ii) the establishment of medical protocols for carrying out diagnoses and monitoring of hearing pathology evolution using methodologies based on Artificial Intelligence. Furthermore, the use of customized hearing aids that allow the application of Rhythmic Auditory Stimulation (RAS) and music therapy enhances audiology as an alternative and innovative way to treat cognitive and degenerative diseases, as well as pathological disorders.
- Does Printing Orientation Matter in PolyJet 3D Printed Teeth for Endodontics? A Micro-CT AnalysisPublication . Barbosa, Cláudia; Reis, Tiago; Reis, José B.; Franco, Margarida; Batista, Catarina; Ruben, Rui B.; Martín-Biedma, Benjamín; Martín-Cruces, JoseThis study aimed to identify the optimal printing orientation (X, Y, or Z axis) and positioning of a mandibular molar presenting an isthmus using PolyJet™ technology. The influence of these parameters on dimensional accuracy and on the behavior of 3D-printed teeth (3DPT) during endodontic preparation with ProTaper Gold® system was evaluated. Six groups (XA, XB, YA, YB, ZA, ZB; n = 10) were printed with different axis orientations and distinct isthmus positions relative to the build platform. All samples underwent micro-computed tomography scanning before and after endodontic preparation. Regarding preoperative analyses—canal volume, centroids, and total tooth volume and area—no significant differences were found between groups XA–YA or XB–YB (p > 0.05), supporting their comparability. In contrast, groups ZA and ZB differed significantly from all others (p < 0.05), failing to meet equivalence required for further comparison, and were therefore excluded. Postoperative evaluation—volume change, centroid displacement, transportation, and unprepared areas—revealed no significant differences between XA–YA and XB–YB. Within the limitations of this study, both printing orientation and position affected the accuracy and repeatability of 3DPT, with positioning exerting the greatest influence, while their behavior towards endodontic preparation remained consistent across orientations.
- Effect of Preventive Exercise Programs for Swimmer’s Shoulder Injury on Rotator Cuff Torque and Balance in Competitive Swimmers: A Randomized Controlled TrialPublication . Tavares, Nuno; Vilas-Boas, João Paulo; Castro, Maria AntónioBackground: Over the season, competitive swimmers experience a progressive imbalance in rotator cuff strength, predisposing them to a significant risk factor for a swimmer’s shoulder injury. Objectives: Verify the effectiveness of two 12-week preventive programs on the shoulder rotators’ peak torque and conventional/functional ratios. Design: A care provider- and participant-blinded, parallel, randomized controlled trial with three groups. Participants: Competitive swimmers aged 16 to 35 years with no prior clinical issues related to their shoulders. Interventions: Twice a week, over 12 weeks, the two experimental groups performed five exercises where the only difference was executing the program with weights or elastic bands, and the control group performed a sham intervention. Main outcome measures: The concentric and eccentric peak torque of the internal and external rotators of the dominant shoulder were assessed before and after the intervention using an isokinetic dynamometer Biodex System 3, at 60°/s, 120°/s, and 180°/s. Results: Among the experimental groups, only one test indicated a reduction (p ≤ 0.05) in rotator peak torque, while the control group showed a decrease (p ≤ 0.05) in five tests. Swimmers who completed the prevention programs demonstrated less imbalance in conventional/functional ratios than controls. Conclusions: Implementing a 12-week preventive program minimizes the progressive shoulder rotational imbalance over the season in competitive swimmers. Clinical Trial Registration number: NCT06552585.
- Effectiveness of Wearable Devices for Posture Correction: A Systematic Review of Evidence from Randomized and Quasi-Experimental StudiesPublication . Caixeiro, Diogo; Cordeiro, Tomás; Constantino, Leandro; Carreira, João; Mendes, Rui; Silva, Cândida G.; Castro, Maria AntónioIntroduction: The increasing development of wearable devices for postural monitoring (provide feedback on posture) or correction (mechanical or biofeedback to promote change) is partly driven by the rising prevalence of poor posture in the general population and its impact on pain perception and functional capacity. Objective: Examine the effects of wearable devices on posture correction or prevention and on related outcomes, including postural alignment, muscle activity, pain and functional performance. Methods: The review followed the PRISMA 2020 guidelines. Searches were performed in PubMed, Scopus, Web of Science, and PEDro for studies published between 2012 and 2025. Eligible studies included randomized controlled trials and quasi-experimental designs involving participants with postural deviations or at risk of developing them, who underwent interventions using wearable devices that provided vibratory, auditory, visual, or tactile biofeedback. Results: Eight studies reported immediate improvements in postural alignment, body awareness, and self-reported pain, particularly with devices providing vibratory or visual biofeedback. Functional task stability improved, and muscle activity during risky postures decreased. However, the strong heterogeneity across devices and protocols, small sample sizes, short intervention durations, and, in some cases, the lack of independent control groups limit the strength and generalizability of these findings. Conclusions: Wearable devices have potential as complementary tools in physiotherapy due to their autonomous and potentially effective nature. Nevertheless, current evidence remains insufficient to support definitive clinical recommendations.
- Effects of Human Mesenchymal Stem Cells Isolated from Wharton’s Jelly of the Umbilical Cord and Conditioned Media on Skeletal Muscle Regeneration Using a Myectomy ModelPublication . Pereira, T.; Armada-da Silva, P.A.S.; Amorim, I.; Rêma, A.; Caseiro, A.R.; Gärtner, A.; Rodrigues, M.; Lopes, M. A.; Bartolo, P.J.; Santos, J.D.; Luís, A.L.; Maurício, A.C.keletal muscle has good regenerative capacity, but the extent of muscle injury and the developed fibrosis might prevent complete regeneration. Thein vivoapplication of human mesenchymal stem cells (HMSCs) of the umbilical cord and the conditioned media (CM) where the HMSCs were cultured and expanded, associated with different vehicles to induce muscle regeneration, was evaluated in a rat myectomy model. Two commercially available vehicles and a spherical hydrogel developed by our research group were used. The treated groups obtained interesting results in terms of muscle regeneration, both in the histological and in the functional assessments. A less evident scar tissue, demonstrated by collagen type I quantification, was present in the muscles treated with HMSCs or their CM. In terms of the histological evaluation performed by ISO 10993-6 scoring, it was observed that HMSCs apparently have a long-term negative effect, since the groups treated with CM presented better scores. CM could be considered an alternative to thein vivotransplantation of these cells, as it can benefit from the local tissue response to secreted molecules with similar results in terms of muscular regeneration. Searching for an optimal vehicle might be the key point in the future of skeletal muscle tissue engineering.
- Electromyographic activity of shoulder muscles on two preventive exercise programmes for swimmer’s shoulder: elastic band versus weightPublication . Tavares, Nuno; Castro, Maria António; Vilas-Boas, João Paulo; Fong, DanielStrength programmes have shown good results in preventing swimmer’s shoulder. However, there aren’t studies based on electromyographic (EMG) analysis in these programmes. This study aims to compare an EMG activity of the middle trapezius (MT), lower trapezius (LT), infraspinatus (IS), serratus anterior (SA), and pectoralis major (PM) in two swimmer’s shoulder preventive programmes – one performed with elastic band and the other with weights. Twenty competitive swimmers performed two strength programmes consisting of five exercises each, while the analysis of EMG activity in MT, LT, IS, SA, and PM for each exercise was recorded. The superficial EMG was used to collect data at a sampling frequency of 1000 hz. The Paired Sample T-test or the Wilcoxon test was applied to compare EMG activity between programmes. The internal rotation at 90º (p < 0.001) and external rotation at 90º (p ≤ 0.01) exercises produced high myoelectric shoulder muscle activity with an elastic band. Conversely, scapular punches (p < 0.001) exercise has high shoulder EMG activity when performed with weights. Performing the same preventive exercise programme with two different instruments produces great variability in the myoelectric activity of the shoulder muscles.
- EMG Activity of the Biceps and Triceps Brachii During Basketball Chest Pass and Reception: Group Differences Based on Age, Experience, and Limb DominancePublication . Amaro, Catarina M.; Castro, Maria António; Amaro, Ana M.Understanding muscle activation patterns during sport-specific skills is essential for optimizing performance and training strategies. In basketball, upper limb actions such as passing and receiving require precise coordination and effective neuromuscular control. The main goal of this study was to analyze and compare the muscle activity of the biceps brachii and triceps brachii during the execution and reception of the two-handed chest pass in basketball players with different levels of competitive experience. Surface electromyography (EMG) data were collected from 14 federated athletes, aged between 11 and 29 years, using the BioSignal Plux system. Participants were allocated into two groups according to their playing experience. Muscle activation was analysed in terms of activation time (AT) and percentage of muscle activation (%MA), normalised to maximum voluntary contraction (MVC). A linear mixed model was used to evaluate the effects of experience level, limb dominance, and their interaction while accounting for repeated measures within participants. No significant differences were observed between dominant and non-dominant limbs for any variable. Significant differences between experience/ age groups were identified mainly in the triceps brachii, particularly for activation time in the lateral head and %MA in the long head. In general, more experienced/aged athletes demonstrated higher levels of neuromuscular activation and shorter activation times, suggesting different motor control strategies. A significant positive association was found between years of practice and %MA of the long head of the triceps brachii. These findings provide novel insights into neuromuscular recruitment during both the execution and reception phases of the basketball chest pass and may inform training strategies aimed at enhancing technical efficiency across developmental stages.
- Expanding the Potential of Self-Assembled Silk Fibroin as Aerogel Particles for Tissue RegenerationPublication . Bernardes, Beatriz G.; Baptista-Silva, Sara; Illanes-Bordomás, Carlos; Magalhães, Rui; Dias, Juliana Rosa; Alves, Nuno M. F.; Costa, Raquel; García-González, Carlos A.; Oliveira, Ana LeiteA newly produced silk fibroin (SF) aerogel particulate system using a supercritical carbon dioxide (scCO2)-assisted drying technology is herein proposed for biomedical applications. Different concentrations of silk fibroin (3%, 5%, and 7% (w/v)) were explored to investigate the potential of this technology to produce size- and porosity-controlled particles. Laser diffraction, helium pycnometry, nitrogen adsorption–desorption analysis and Fourier Transform Infrared with Attenuated Total Reflectance (FTIR-ATR) spectroscopy were performed to characterize the physicochemical properties of the material. The enzymatic degradation profile of the SF aerogel particles was evaluated by immersion in protease XIV solution, and the biological properties by cell viability and cell proliferation assays. The obtained aerogel particles were mesoporous with high and concentration dependent specific surface area (203–326 m2/g). They displayed significant antioxidant activity and sustained degradation in the presence of protease XIV enzyme. The in vitro assessment using human dermal fibroblasts (HDF) confirm the particles’ biocompatibility, as well as the enhancement in cell viability and proliferation.
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