ESTG - Comunicações em conferências e congressos internacionais
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Percorrer ESTG - Comunicações em conferências e congressos internacionais por Domínios Científicos e Tecnológicos (FOS) "Ciências Naturais::Ciências Biológicas"
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- An Injectable Thermoreversible Hydrogel Loaded with ManNAc for GNE MyopathyPublication . Violante, Cristiana; Marques-da-Silva, Dorinda; Videira, Paula Alexandra; Lagoa, RicardoGNE myopathy (GNEM) is a rare adult-onset disease characterized by atrophy and weakness of skeletal muscles caused by mutations in the GNE gene. There is no approved therapy, but clinical trials with supplementation with N-acetylmannosamine (ManNAc) alleviated the GNEM-associated decrease in sialylation. Nevertheless, oral intake of ManNAc has shown low absorption and high gastrointestinal adverse effects. In this regard, the aim of this work was to explore an alternative delivery route for ManNAc directly targeting the muscle tissues. For this we investigated the thermal responses of Pluronic F-127 gel and its muscle adhesion after subcutaneous injection, and the kinetics of release of gelencapsulated ManNAc in phosphate buffered saline medium. The results obtained show the thermal response of Pluronic F-127 represents a unique opportunity for its injectability and function as a drug delivery system directly acting on the muscle surrounding area and showing gel stability in the target area. Moreover, the continuous delivery of ManNAc from the gel (20% Pluronic F-127) was detected during 3h, at a continuous rate and slower than using a simple ManNAc solution. In conclusion, the data obtained in this work supports the possible use of the Pluronic F-127 thermoreversible hydrogel loaded with ManNAc in GNE myopathy therapy and research.
- Temporal Anomaly Detection: An Artificial Immune Approach Based on T-Cell Activation, Clonal Size Regulation and HomeostasisPublication . Antunes, Mário J.; Correia, Manuel E.This paper presents an artificial immune system (AIS) based on Grossman's tunable activation threshold (TAT) for temporal anomaly detection. We describe the generic AIS framework and the TAT model adopted for simulating T Cells behaviour, emphasizing two novel important features: the temporal dynamic adjustment of T Cells clonal size and its associated homeostasis mechanism. We also present some promising results obtained with artificially generated data sets, aiming to test the appropriateness of using TAT in dynamic changing environments, to distinguish new unseen patterns as part of what should be detected as normal or as anomalous. We conclude by discussing results obtained thus far with artificially generated data sets.