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Percorrer Escola de Tecnologia e Gestão por Domínios Científicos e Tecnológicos (FOS) "Ciências Agrárias::Agricultura, Silvicultura e Pescas"
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- 2-Hydroxy-4-methoxybenzaldehyde fromHemidesmus indicusis antagonistic toStaphylococcus epidermidisbiofilm formationPublication . Kannappan, Arunachalam; Durgadevi, Ravindran; Srinivasan, Ramanathan; Lagoa, Ricardo José Lucas; Packiavathy, Issac Abraham Sybiya Vasantha; Pandian, Shunmugiah Karutha; Ravi, Arumugam VeeraStaphylococcus epidermidis (SE) is an opportunistic nosocomial pathogen that accounts for recalcitrant device-related infections worldwide. Owing to the growing interest in plants and their secondary metabolites targeting bacterial adhesion, this study was intended to uncover the anti-biofilm potential of Hemidesmus indicus and its major constituent 2-hydroxy-4-methoxybenzaldehyde (HMB) against SE. The minimum biofilm inhibitory concentration (MBIC) of H. indicus root extract and HMB were found to be 500 and 250 µg ml−1, respectively. The results of time-dependent biofilm inhibition and mature biofilm disruption assays confirmed that HMB targets initial cell adhesion. Furthermore, interference by HMB in the expression of adhesin genes (icaA, aap and bhp) and biofilm components was associated with an increased susceptibility of SE to oxidative stress and antibiotics. To conclude, this study reports for the first time HMB as a potential drug against SE biofilms.
- Disentangling drought-induced variation in ecosystem and soil respiration using stable carbon isotopesPublication . Unger, Stephan; Máguas, Cristina; Pereira, João S.; Aires, Luis M.; David, Teresa S.; Werner, ChristianeCombining C flux measurements with information on their isotopic composition can yield a process-based understanding of ecosystem C dynamics. We studied the variations in both respiratory fluxes and their stable C isotopic compositions (δ13C) for all major components (trees, understory, roots and soil microorganisms) in a Mediterranean oak savannah during a period with increasing drought. We found large drought-induced and diurnal dynamics in isotopic compositions of soil, root and foliage respiration (δ13Cres). Soil respiration was the largest contributor to ecosystem respiration (Reco), exhibiting a depleted isotopic signature and no marked variations with increasing drought, similar to ecosystem respired δ13CO2, providing evidence for a stable C-source and minor influence of recent photosynthate from plants. Short-term and diurnal variations in δ13Cres of foliage and roots (up to 8 and 4‰, respectively) were in agreement with: (1) recent hypotheses on post-photosynthetic fractionation processes, (2) substrate changes with decreasing assimilation rates in combination with increased respiratory demand, and (3) decreased phosphoenolpyruvate carboxylase activity in drying roots, while altered photosynthetic discrimination was not responsible for the observed changes in δ13Cres. We applied a flux-based and an isotopic flux-based mass balance, yielding good agreement at the soil scale, while the isotopic mass balance at the ecosystem scale was not conserved. This was mainly caused by uncertainties in Keeling plot intercepts at the ecosystem scale due to small CO2 gradients and large differences in δ13Cres of the different component fluxes. Overall, stable isotopes provided valuable new insights into the drought-related variations of ecosystem C dynamics, encouraging future studies but also highlighting the need of improved methodology to disentangle short-term dynamics of isotopic composition of Reco.
- Drones for litter mapping: An inter-operator concordance test in marking beached items on aerial imagesPublication . Andriolo, Umberto; Gonçalves, Gil; Rangel-Buitrago, Nelson; Paterni, Marco; Bessa, Filipa; Gonçalves, Luisa M. S.; Sobral, Paula; Bini, Monica; Duarte, Diogo; Fontán-Bouzas, Ángela; Gonçalves, Diogo; Kataoka, Tomoya; Luppichini, Marco; Pinto, Luis; Topouzelis,Konstantinos; Vélez-Mendoza, Anubis; Merlino, SilviaUnmanned aerial systems (UAS, aka drones) are being used to map macro-litter on the environment. Sixteen qualified researchers (operators), with different expertise and nationalities, were invited to identify, mark and categorize the litter items (manual image screening, MS) on three UAS images collected at two beaches. The coefficient of concordance (W) among operators varied between 0.5 and 0.7, depending on the litter parameter (type, material and colour) considered. Highest agreement was obtained for the type of items marked on the highest resolution image, among experts in litter surveys (W = 0.86), and within territorial subgroups (W = 0.85). Therefore, for a detailed categorization of litter on the environment, the MS should be performed by experienced and local operators, familiar with the most common type of litter present in the target area. This work provides insights for future operational improvements and optimizations of UAS-based images analysis to survey environmental pollution.
- Dynamics of Epidemiological ModelsPublication . Pinto, Alberto; Aguiar, Maíra; Martins, José; Stollenwerk, NicoWe study the SIS and SIRI epidemic models discussing different approaches to compute the thresholds that determine the appearance of an epidemic disease. The stochastic SIS model is a well known mathematical model, studied in several contexts. Here, we present recursively derivations of the dynamic equations for all the moments and we derive the stationary states of the state variables using the moment closure method. We observe that the steady states give a good approximation of the quasi-stationary states of the SIS model. We present the relation between the SIS stochastic model and the contact process introducing creation and annihilation operators. For the spatial stochastic epidemic reinfection model SIRI, where susceptibles S can become infected I, then recover and remain only partial immune against reinfection R, we present the phase transition lines using the mean field and the pair approximation for the moments. We use a scaling argument that allow us to determine analytically an explicit formula for the phase transition lines in pair approximation.
- Effectiveness of Nature-Based Solutions in Mitigating Flood Hazard in a Mediterranean Peri-Urban CatchmentPublication . Ferreira, Carla S.; Mourato, Sandra; Kasanin-Grubin, Milica; Ferreira, António J. D.; Destouni, Georgia; Kalantari, ZahraUrbanization alters natural hydrological processes and enhances runoff, which affects flood hazard. Interest in nature-based solutions (NBS) for sustainable mitigation and adaptation to urban floods is growing, but the magnitudes of NBS effects are still poorly investigated. This study explores the potential of NBS for flood hazard mitigation in a small peri-urban catchment in central Portugal, prone to flash floods driven by urbanization and short but intense rainfall events typical of the Mediterranean region. Flood extent and flood depth are assessed by manually coupling the hydrologic HEC-HMS and hydraulic HEC-RAS models. The coupled model was run for single rainfall events with recurrence periods of 10−, 20−, 50−, and 100−years, considering four simulation scenarios: current conditions (without NBS), and with an upslope NBS, a downslope NBS, and a combination of both. The model-simulation approach provides good estimates of flood magnitude (NSE = 0.91, RMSE = 0.08, MAE = 0.07, R2 = 0.93), and shows that diverting streamflow into abandoned fields has positive impacts in mitigating downslope flood hazard. The implementation of an upslope NBS can decrease the water depth at the catchment outlet by 0.02 m, whereas a downslope NBS can reduce it from 0.10 m to 0.23 m for increasing return periods. Combined upslope and downslope NBS have a marginal additional impact in reducing water depth, ranging from 0.11 m to 0.24 m for 10− and 100−year floods. Decreases in water depth provided by NBS are useful in flood mitigation and adaptation within the peri-urban catchment. A network of NBS, rather than small isolated strategies, needs to be created for efficient flood-risk management at a larger scale.
- The effectiveness of past wildfire at limiting reburning is short-lived in a Mediterranean humid climatePublication . Davim, David A.; Rossa, Carlos G.; Pereira, José M. C.; Guiomar, Nuno; Fernandes, Paulo M.Background: The study of wildfire interactions (i.e., spread limitation and reburns) is gaining traction as a means of describing the self-limiting process of fire spread in the landscape and has important management implications but has scarcely been attempted in Europe. We examined to what extent previously burned areas restricted the development of individual large wildfires (> 500 ha) in mainland Portugal. Results: For the 1984–2021 period, we (1) modeled the proportion of large wildfire perimeters coinciding with transitions to shorter time since fire (TSF), i.e., locations where fire spread ceased upon encountering assumedly less flammable fuels, and (2) characterized the prevalence of different TSF in the composition of the area burned by large wildfires in relation to available TSF. Only 4% of the large wildfires did not comprise edges intersecting past wildfires. Low TSF (especially up to 8 years) resulted in large-wildfire perimeter limitation at TSF transitions. This effect was further enhanced by high historical burn probability and proximity to roadways and watercourses. Perimeter limitation did also increase under high (but not very high or extreme) fire danger, benefiting from maximum seasonal firefighting preparedness. TSF prevalence in the composition of large-wildfire area was extremely variable and thus an overall weak pattern emerged, with minimum and maximum prevalence respectively at TSF < 2 years and TSF ≥ 6 years. Conclusions: Large wildfire limitation in Portugal is hampered by fast fuel build-up after fire, indicating a short-lived fire-hazard reduction effect under the prevailing Mediterranean humid climate of the study region. Nonetheless, such effect should be considered when planning fuel-reduction treatments and can be used opportunistically during large-wildfire suppression operations.
- Nanofibrillated cellulose originated from Rhododendron ponticum to produce scaffolds using 3D printing for biomedical applicationsPublication . Lima, Tielidy A. de M. de; Lima, Gabriel Goetten de; Munir, Nimra; Coutinho, Joana Raquel Teixeira; Mitchell, Geoffrey Robert; Magalhães, Washington L. E.; Nugent, Michael J. D.Rhododendron ponticum is an invasive species that spreads rapidly and is described as one of the biggest threats to peatlands in Ireland. This study offers an innovative approach to utilizing Rhododendron waste. Initially, sawdust was submitted to a bleaching treatment and the nanofibrillated cellulose (NFC) was obtained using two different methods: ultra-fine friction grinding and twin-screw extrusion with the assistance of TEMPO (2,2,6,6-tetramethyl-1-piperidinyloxy) pre-treatment. The samples processed through twin-screw extrusion exhibited the presence of NFC at five intervals, as confirmed by TEM analysis. However, these samples displayed a higher diameter deviation compared to those processed through grinding alone. Notably, after 20 extrusion steps, the NFC diameter became more uniform, reaching approximately 35 nm. Sedimentation tests showed that extrusion produced more homogeneous cellulose size than the grinder method. However, FTIR characterization for the samples showed a unique band related to C-O-C glycosidic linkage. The results showed that grinding breaks these groups resulting in crystallinity values lower than extrusion, 50 % compared 60 %. Therefore, NFC with 20 steps by grinding was blended with polycaprolactone to produce a 3D scaffold using a 3D printer at different ratios of 1–5 % addition. The effect of 1 % of NFC was unique showing significant enhanced mechanical properties compared to pure polycaprolactone (PCL), additionally, the NFC does not exhibit toxicity so these materials show promise for biomedical applications.
- Plum Ripeness Analysis in Real Environments Using Deep Learning with Convolutional Neural NetworksPublication . Miragaia, Rolando; Chávez, Francisco; Díaz, Josefa; Vivas, Antonio; Prieto, Maria Henar; Moñino, Maria JoséDigitization and technological transformation in agriculture is no longer something of the future, but of the present. Many crops are being managed by using sophisticated sensors that allow farmers to know the status of their crops at all times. This modernization of crops also allows for better quality harvests as well as significant cost savings. In this study, we present a tool based on Deep Learning that allows us to analyse different varieties of plums using image analysis to identify the variety and its ripeness status. The novelty of the system is the conditions in which the designed algorithm can work. An uncontrolled photographic acquisition method has been implemented. The user can take a photograph with any device, smartphone, camera, etc., directly in the field, regardless of light conditions, focus, etc. The robustness of the system presented allows us to differentiate, with 92.83% effectiveness, three varieties of plums through images taken directly in the field and values above 94% when the ripening stage of each variety is analyzed independently. We have worked with three varieties of plums, Red Beaut, Black Diamond and Angeleno, with different ripening cycles. This has allowed us to obtain a robust classification system that will allow users to differentiate between these varieties and subsequently determine the ripening stage of the particular variety.
- Production of glued laminated timber with copper azole treated maritime pinePublication . Gaspar, Florindo; Cruz, Helena; Gomes, Augusto; Nunes, LinaA study was conducted to evaluate the performance of glued laminated timber (glulam) of maritime pine treated with a copper azole preservative product. Shear strength of glue lines met the requirements of EN 386 (2001) with no influence of clamping pressure and cure temperature. According to the same standard, delamination was satisfactory for higher cure temperatures applied with a clamping pressure of 0.6 N/mm2. Finger joints made with treated wood gave satisfactory bending strength. The preservative treatment did not influence the modulus of elasticity of the beams. According to a Monte Carlo simulation and following the requirements of EN 1194 (2002), glulam of class GL 28c can be manufactured if visually graded maritime pine of classes E and EE (NP 4305 1995) is used in the inner and outer lamellas, respectively, and class GL 24h when using both grades in equal proportions randomly distributed through the glulam element.
- A stochastic approach to optimize Maritime pine (Pinus pinaster Ait.) stand management scheduling under fire risk. An application in PortugalPublication . Ferreira, L.; Constantino, M.; Borges, J. G.The paper discusses research aiming at the development of a management scheduling model for even-aged stands that may take into consideration fuel treatments to address the risk of wildfires. A Stochastic dynamic programming (SDP) approach is proposed to determine the policy (e.g. the fuel treatment and thinning schedules and the rotation age) that produces the maximum expected discounted net revenue. Fuel treatment activities encompass shrub cleanings. Emphasis was on combining a deterministic stand-level growth and yield model with wildfire occurrence and damage models to design a SDP network. SDP stages are defined by age and state variables include both the stand basal area and the number of years since the last fuel treatment. Fire occurrence and damage scenarios are addressed at each stage. Results from an application to Maritime pine (Pinus pinaster Ait.) stand management scheduling in Leiria National Forest, Portugal, are presented. Results suggest that the modeling strategy may help assess the impact of wildfire risk on the optimal stand management schedule. They confirm that the maximum expected discounted net revenues decreases. Further, albeit some timber may be salvaged after the wildfire, rotation age also decreases when the risk of fire is considered. Finally, they provide interesting insights about the role of thinning and fuel treatment policies in mitigating risk.